stro 1 pe  (Santa Cruz Biotechnology)

Journal: Cells

Article Title: Inflammatory Modulation of Toll-like Receptors in Periodontal Ligament Stem Cells: Implications for Periodontal Therapy

doi: 10.3390/cells14060432

Figure Lengend Snippet: Detailed list of antibodies and isotype controls showing manufacturers, fluorophore labels, and catalog numbers.

Article Snippet: STRO-1 PE , Santa Cruz Biotechnology, Dallas, TX, USA , sc-47733.

Techniques: Control

Journal: Mediators of Inflammation

Article Title: circRNA CDR1as Regulated the Proliferation of Human Periodontal Ligament Stem Cells under a Lipopolysaccharide-Induced Inflammatory Condition

doi: 10.1155/2019/1625381

Figure Lengend Snippet: Isolation and identification of PDLSCs. (a) Cells isolated from periodontal ligament tissue show a long and spindle-shaped morphology under phase-contrast microscopy. (b, c) The isolated cells were negative for CD34 and CD45 presented as histogram of flow cytometry analysis. (d) Dot plots represent typical examples of STRO-1 expression and exhibited 45% of STRO-1 positive expression analyzed by flow cytometry. (e) The purity of the sorted population was >96% as revealed by postresorting analysis.

Article Snippet: Harvested cells were incubated with human MSC positive maker of STRO-1-PE (BD Pharmingen) for 20 min on ice in the dark, followed by washing and resuspension in HBSS/2% FBS containing 1 mg/ml 7-AAD.

Techniques: Isolation, Microscopy, Flow Cytometry, Expressing